| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048658 |
| Chromosome: | chromosome 3 |
| Location: | 3971812 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171751 | (1 of 5) K16302 - metal transporter CNNM (CNNM) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTGCACAAAGTCCCGCCCACCCCACCCA |
| Internal bar code: | CGCATGGGGCGTCAGTAGCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 85.4586 |
| LEAP-Seq n confirming: | 382 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGCTCTTCAGCAAACACC |
| Suggested primer 2: | CTCGAGGAACTCAGACCAGG |