| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048689 |
| Chromosome: | chromosome 3 |
| Location: | 2002206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155950 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS/intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTACGCTAGGGCGTGACACGGCGCCGGGGA |
| Internal bar code: | ACATACTTTTCTACGCAAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 92.1053 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACAAACGGTCCAAAAGT |
| Suggested primer 2: | CGGTTCGGGTTGAAGACTAA |