Insertion junction: LMJ.RY0402.048700_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048700
Chromosome:chromosome 10
Location:6379871
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g465650 (1 of 2) IPR001680//IPR011047//IPR017986//IPR020472//IPR027417 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain // G-protein beta WD-40 repeat // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCCCCACCCCTCAACCGCCCCTCACCCC
Internal bar code:CTTATTCACGGGTTGGCGTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:180
LEAP-Seq percent confirming:67.4249
LEAP-Seq n confirming:741
LEAP-Seq n nonconfirming:358
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGGACATGATCCTGGACGAC
Suggested primer 2:GCGCCTCCTCCAAATACATA