| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048730 |
| Chromosome: | chromosome 6 |
| Location: | 605144 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g253300 | UBCH1 | (1 of 1) K05610 - ubiquitin carboxyl-terminal hydrolase L5 [EC:3.4.19.12] (UCHL5, UCH37) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACTCCGACGACACACGTCCTCACCGACA |
| Internal bar code: | CTGTGGACGCGCATCTACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1172 |
| LEAP-Seq percent confirming: | 99.659 |
| LEAP-Seq n confirming: | 7599 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAGTTGCCATGGTATGCG |
| Suggested primer 2: | CGCTTTCCGTCTTGACTTTC |