Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.048734 |
Chromosome: | chromosome 7 |
Location: | 5082385 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g347600 | (1 of 1) PTHR11059:SF0 - DNA REPAIR PROTEIN RECN | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCTCACGCTGCGACCCTCACGCTCTGC |
Internal bar code: | ACTCCCCTCACGTGGCCGTTGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 960 |
LEAP-Seq percent confirming: | 98.7512 |
LEAP-Seq n confirming: | 5219 |
LEAP-Seq n nonconfirming: | 66 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TACATCCAGACGACGAGCTG |
Suggested primer 2: | ACAACAACAGCACAGCGAAC |