Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.048748 |
Chromosome: | chromosome 7 |
Location: | 1768328 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g325720 | (1 of 5) IPR000104//IPR001054//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAAGGGTAGGGCATCACACTTTACGAG |
Internal bar code: | TTTCCACATACGCATCGTTCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 982 |
LEAP-Seq percent confirming: | 98.3575 |
LEAP-Seq n confirming: | 11138 |
LEAP-Seq n nonconfirming: | 186 |
LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTGTCCAGGCTAGGGACTTG |
Suggested primer 2: | TGTCAAGCACAAGCACAACA |