| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048770 |
| Chromosome: | chromosome_6 |
| Location: | 5360230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g283550 | CRC2 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACCGCCCTCAGTTGCGCTGCTTCCCAGC |
| Internal bar code: | TGGCGATTTGGTGGACCACAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 818 |
| LEAP-Seq percent confirming: | 97.624 |
| LEAP-Seq n confirming: | 945 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGACGCTGAAGACATATCA |
| Suggested primer 2: | AACTGGACTTCCAGGTGTGG |