Insertion junction: LMJ.RY0402.048775_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048775
Chromosome:chromosome 12
Location:5207813
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g527918 (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTGGGCCAACACGCGTTGGAGAGCGCGG
Internal bar code:TGGAACCGGCGTACCAGAAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AAATACGTGTGCGACCATCA
Suggested primer 2:CGGCTGCATATGGTTGTATG