| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048798 |
| Chromosome: | chromosome 9 |
| Location: | 2299744 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392300 | NAT22 | N-acetyltransferase; (1 of 3) IPR000182//IPR011011//IPR016181 - GNAT domain // Zinc finger, FYVE/PHD-type // Acyl-CoA N-acyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAGTTTGGTCCGTGGGGAAGGGTGCG |
| Internal bar code: | CTGCGCCTAAGTAGGTCGTAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 460 |
| LEAP-Seq percent confirming: | 99.7986 |
| LEAP-Seq n confirming: | 12883 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGTTGGCCGTGGAACTG |
| Suggested primer 2: | CGCAGGGTGTAGTACCGAAT |