Insertion junction: LMJ.RY0402.048798_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048798
Chromosome:chromosome 9
Location:2299944
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g392300 NAT22 N-acetyltransferase; (1 of 3) IPR000182//IPR011011//IPR016181 - GNAT domain // Zinc finger, FYVE/PHD-type // Acyl-CoA N-acyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGACCATGGCGGAACTTCCCAGTCATACC
Internal bar code:GGACCGTAAGCACTGTTGTCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:721
LEAP-Seq percent confirming:93.2252
LEAP-Seq n confirming:3564
LEAP-Seq n nonconfirming:259
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACCTACCGTCAATTCGT
Suggested primer 2:GAGTCCAGAGATTTCGTCGC