| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048799 |
| Chromosome: | chromosome 9 |
| Location: | 2041073 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394250 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGGAACACCTGCGCCGCGTTGTCCTCG |
| Internal bar code: | GATTGGGTGGGTTTGCCCGAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 316 |
| LEAP-Seq percent confirming: | 99.5736 |
| LEAP-Seq n confirming: | 467 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGCGCTATCCAGCACAC |
| Suggested primer 2: | GACCACATCACACTCATGGC |