| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048809 |
| Chromosome: | chromosome 1 |
| Location: | 1525870 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g007901 | (1 of 3) PTHR11206//PTHR11206:SF95 - MULTIDRUG RESISTANCE PROTEIN // MULTI ANTIMICROBIAL EXTRUSION FAMILY PROTEIN | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCTGAACCTGTTGTGCAGGCGTGACCCA |
| Internal bar code: | CCTTATGCTGCGACTGCTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 97.0588 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGTATTTTGCGGTGTACT |
| Suggested primer 2: | GAATGTGTGGTACGTGTCGG |