Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.048811 |
Chromosome: | chromosome 2 |
Location: | 6325441 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g115050 | RSEP3,RSE3 | (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAAGGCCAGTCCGCCCAGCGGCCACAG |
Internal bar code: | CGTGTACACAGGTGGCTGACCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 753 |
LEAP-Seq percent confirming: | 98.1538 |
LEAP-Seq n confirming: | 319 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTGCTCAGGGAAGGAGTGAG |
Suggested primer 2: | TCTTTTCCATTCCCATCGAG |