| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048811 |
| Chromosome: | chromosome_2 |
| Location: | 6325441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g115050 | RSE3,RSEP3 | Intramembrane metalloprotease | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATGAAGGCCAGTCCGCCCAGCGGCCACAG |
| Internal bar code: | CGTGTACACAGGTGGCTGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 753 |
| LEAP-Seq percent confirming: | 98.1538 |
| LEAP-Seq n confirming: | 319 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCTCAGGGAAGGAGTGAG |
| Suggested primer 2: | TCTTTTCCATTCCCATCGAG |