Insertion junction: LMJ.RY0402.048811_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.048811
Chromosome:chromosome 2
Location:6325441
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre02.g115050 RSEP3,RSE3 (1 of 3) 3.4.24.85 - S2P endopeptidase / Sterol-regulatory element-binding proteins intramembrane protease; Intramembrane metalloprotease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATGAAGGCCAGTCCGCCCAGCGGCCACAG
Internal bar code:CGTGTACACAGGTGGCTGACCG

Confirmation - LEAP-Seq

LEAP-Seq distance:753
LEAP-Seq percent confirming:98.1538
LEAP-Seq n confirming:319
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTGCTCAGGGAAGGAGTGAG
Suggested primer 2:TCTTTTCCATTCCCATCGAG