| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048821 |
| Chromosome: | chromosome 1 |
| Location: | 5618622 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039900 | TRZ4 | RNase Z-like protein; (1 of 3) K00784 - ribonuclease Z (rnz) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGTTGTCTTGTTGGGGTAGTCGAAAGCA |
| Internal bar code: | TCACCGATGCGATACCGGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 783 |
| LEAP-Seq percent confirming: | 99.3671 |
| LEAP-Seq n confirming: | 628 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGTGCTGGTCAAGTACA |
| Suggested primer 2: | GGAGAAGTGGGTCAGGATGA |