| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048823 |
| Chromosome: | chromosome 12 |
| Location: | 2960943 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501403 | MLO2 | (1 of 1) PF03094//PF13833 - Mlo family (Mlo) // EF-hand domain pair (EF-hand_8); MLO family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGTGGCGGCCATAGGCGCAGCTGCGT |
| Internal bar code: | GTTTAATAACGGTTTCGGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 211 |
| LEAP-Seq percent confirming: | 99.4575 |
| LEAP-Seq n confirming: | 550 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGCTAGCCAACCGAGTCAA |
| Suggested primer 2: | GGGTCGTTTTTGCAGTTTGT |