Insertion junction: LMJ.RY0402.048850_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048850
Chromosome:chromosome 4
Location:1839132
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre04.g211650 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACACGCCACTGACAACTAACAACCGCA
Internal bar code:GGGCAGGAGTGACGATCGGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:431
LEAP-Seq percent confirming:86.5106
LEAP-Seq n confirming:6022
LEAP-Seq n nonconfirming:939
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGAGGGGATGATAGTGAT
Suggested primer 2:CCTTCAGAATCTGTCGAGGC