Insertion junction: LMJ.RY0402.048850_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.048850
Chromosome:chromosome 12
Location:5872087
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g533800 CYN22 Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAGCACGGAGCTCATGCCCACGCGCACAC
Internal bar code:TGTACGACGGGCGAGTATAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:945
LEAP-Seq percent confirming:99.6378
LEAP-Seq n confirming:11554
LEAP-Seq n nonconfirming:42
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGAGTGTTGGGGTCTGG
Suggested primer 2:TCGTGGCTGTTTATGCTGTC