| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.048858 |
| Chromosome: | chromosome 13 |
| Location: | 1871017 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575550 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCAACGTGTGTGCGCACTACGGCAAAG |
| Internal bar code: | TCGGCGGTCCGGGCCTGCGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1032 |
| LEAP-Seq percent confirming: | 87.0034 |
| LEAP-Seq n confirming: | 5168 |
| LEAP-Seq n nonconfirming: | 772 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAATAACTGCCGCCAACG |
| Suggested primer 2: | GCATGGTGGTTTGACTTGTG |