Insertion junction: LMJ.RY0402.048865_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.048865
Chromosome:chromosome 17
Location:2701165
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g717950 PHC28,PHC6 (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Pherophorin-chlamydomonas homolog 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAACAGGTCGCCTGTGATAGGCTGTGCG
Internal bar code:ATGGCCCCGGGGTTGCCCGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:406
LEAP-Seq percent confirming:99.833
LEAP-Seq n confirming:2391
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCTGGTAACCCCTCAACA
Suggested primer 2:CGTTTCGTCTTGGTCCAAAT