| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048875 |
| Chromosome: | chromosome 3 |
| Location: | 5284323 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183550 | (1 of 3) PTHR15160//PTHR15160:SF4 - VON HIPPEL-LINDAU PROTEIN // PROTEIN F23H11.5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGTCTGGGTGCCCCTTCTACGTTGCTCAG |
| Internal bar code: | TAGACTCATAGTCGGGGTCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 639 |
| LEAP-Seq percent confirming: | 99.8214 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGGTGGACATGGTTGGGA |
| Suggested primer 2: | CCCTCTCTTTCTGCTTGTGG |