Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.048928 |
Chromosome: | chromosome_9 |
Location: | 3322392 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g388393 | ELG13 | Exostosin-like glycosyltransferase | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGTTTCGCCTGACTGTTGTGGTGACCCCGC |
Internal bar code: | GAGGCCTAGATCGGTGGGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 116 |
LEAP-Seq percent confirming: | 98.9691 |
LEAP-Seq n confirming: | 2112 |
LEAP-Seq n nonconfirming: | 22 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATAAGCAGGCAGATGGATGG |
Suggested primer 2: | TCACTTTGGCTCGACTGTTG |