| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048934 |
| Chromosome: | chromosome 16 |
| Location: | 6035303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676350 | (1 of 1) PTHR23073//PTHR23073:SF37 - 26S PROTEASE REGULATORY SUBUNIT // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATAAGGGGCATGCGTGAGTGCAGTGAT |
| Internal bar code: | GGTCGTCGCTAGGTTGGGGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 786 |
| LEAP-Seq percent confirming: | 96.75 |
| LEAP-Seq n confirming: | 387 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGAAGCCAGCCTTTACACG |
| Suggested primer 2: | CCATGGGATGGTGTGTATCA |