| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048938 |
| Chromosome: | chromosome 3 |
| Location: | 2556274 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g160300 | RAM1,RAMP4 | Ribosome-associated membrane protein, ER-stress-associated; (1 of 1) PF06624 - Ribosome associated membrane protein RAMP4 (RAMP4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGCGGTAGGAGTCCCATGCAGAGCTCAT |
| Internal bar code: | TTTGCTCTTGGCGAGGCTCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 963 |
| LEAP-Seq percent confirming: | 99.1979 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCAGAAGCATCCAAGGAC |
| Suggested primer 2: | TGTGCCCAAGATCAAAATCA |