| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048952 |
| Chromosome: | chromosome 12 |
| Location: | 8275691 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550152 | (1 of 1) PF12253 - Chromatin assembly factor 1 subunit A (CAF1A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGATGGAGGAGCGGCATATTGATGTGC |
| Internal bar code: | CAGCGGTCGCTCTGACCACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 869 |
| LEAP-Seq percent confirming: | 98.9939 |
| LEAP-Seq n confirming: | 15841 |
| LEAP-Seq n nonconfirming: | 161 |
| LEAP-Seq n unique pos: | 83 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCTGAACGAGATAACGA |
| Suggested primer 2: | CCCCCAGAAAGCATGTAAGA |