Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.048952 |
Chromosome: | chromosome 12 |
Location: | 8275699 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g550152 | (1 of 1) PF12253 - Chromatin assembly factor 1 subunit A (CAF1A) | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCGACAGCCATCTGCTACTGGTGCTGC |
Internal bar code: | TGTAGGTCAATCTTCCGTAGAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 465 |
LEAP-Seq percent confirming: | 97.4754 |
LEAP-Seq n confirming: | 8726 |
LEAP-Seq n nonconfirming: | 226 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGTGCTGAACGAGATAACGA |
Suggested primer 2: | CCCCCAGAAAGCATGTAAGA |