| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048953 |
| Chromosome: | chromosome 9 |
| Location: | 1961886 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394900 | CYA10 | (1 of 1) IPR000209//IPR001054//IPR029787 - Peptidase S8/S53 domain // Adenylyl cyclase class-3/4/guanylyl cyclase // Nucleotide cyclase; Solute-binding protein-like adenylate cyclase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCACCACGTGAGCTAGTGAAGAATAGT |
| Internal bar code: | TCCTCAATCTTGTGCCTCGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.698 |
| LEAP-Seq n confirming: | 14856 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAAGAAGAGGCGCTGTTG |
| Suggested primer 2: | GCAGGCTGCAAGACATGTAA |