Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.048955 |
Chromosome: | chromosome 9 |
Location: | 6430765 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
intergenic |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTCCCCTTCCATGTGCATGTCACACCCCC |
Internal bar code: | CGTCAGAAATCCGTCCGTCGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 334 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 824 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CTCTCGACCTTAGGATCCCC |
Suggested primer 2: | TGGAACCTGTCTCCCTGAAC |