| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048961 |
| Chromosome: | chromosome 11 |
| Location: | 516869 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467598 | (1 of 1) IPR000104//IPR013763 - Antifreeze protein, type I // Cyclin-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCAGGGTGTGTTTGGGGCCTTCCACAGC |
| Internal bar code: | CGGGGATCCATCGGCCGGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 99.789 |
| LEAP-Seq n confirming: | 946 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGATGCAATCAATCCCTT |
| Suggested primer 2: | TGCCGTATTTTAAACGGAGG |