| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.048970 |
| Chromosome: | chromosome 15 |
| Location: | 1331812 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g641926 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATACCGTCACATGTACCTGCCTGTTTG |
| Internal bar code: | GGCGGTGGGGGGTATGTAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 69 |
| LEAP-Seq percent confirming: | 85.5932 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCATTCGTTCCGTTGAT |
| Suggested primer 2: | AAGGCGGCAGGTAGATAGGT |