| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.048974 |
| Chromosome: | chromosome 9 |
| Location: | 3658032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390541 | (1 of 4) 3.1.1.23 - Acylglycerol lipase / Monoacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCATCCCCGACTTCGACGACACTCGCTTG |
| Internal bar code: | TCACGCAATCTCAGGGGCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 900 |
| LEAP-Seq percent confirming: | 99.4622 |
| LEAP-Seq n confirming: | 9617 |
| LEAP-Seq n nonconfirming: | 52 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGGTTACTCATCCCCC |
| Suggested primer 2: | TTGGACACTTTGTTGGGACA |