| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048974 |
| Chromosome: | chromosome 9 |
| Location: | 3658043 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g390541 | (1 of 4) 3.1.1.23 - Acylglycerol lipase / Monoacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGTTCGGGGCAGAACTTCCACAAAAGC |
| Internal bar code: | ACGGAAGGCTGTACGACGGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 873 |
| LEAP-Seq percent confirming: | 99.5636 |
| LEAP-Seq n confirming: | 1597 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGAGGTTACTCATCCCCC |
| Suggested primer 2: | TTGGACACTTTGTTGGGACA |