| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.048992 |
| Chromosome: | chromosome 6 |
| Location: | 1464871 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259500 | CYG2 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTCTGCTTTTCTACTTCCTCCTCGCAAT |
| Internal bar code: | TTCTCCTGGGCGTTCCCGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 64.6067 |
| LEAP-Seq n confirming: | 230 |
| LEAP-Seq n nonconfirming: | 126 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCTGTATGTCGCTTGCTG |
| Suggested primer 2: | CTGCTGCTGCTGTCTTTGTC |