| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049008 |
| Chromosome: | chromosome 8 |
| Location: | 2550920 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g372400 | UBC19 | E2 Ubiquitin conjugating enzyme; (1 of 1) K04554 - ubiquitin-conjugating enzyme E2 J2 (UBE2J2, NCUBE2, UBC6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTCCGGTTCAATGTTGGCCACGCAGGAC |
| Internal bar code: | GGTCTAACGGTGCTGTCCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 517 |
| LEAP-Seq percent confirming: | 98.8879 |
| LEAP-Seq n confirming: | 1067 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCTTTGGAGCTGATTGG |
| Suggested primer 2: | ACATCCTCCTTCCACATTGC |