| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049040 |
| Chromosome: | chromosome 5 |
| Location: | 3257154 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240200 | (1 of 5) IPR001841//IPR013083 - Zinc finger, RING-type // Zinc finger, RING/FYVE/PHD-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGCGCACAAATCGTTCATTTGGGCAGCT |
| Internal bar code: | CTAGTGGGTGAGTCCTACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 736 |
| LEAP-Seq percent confirming: | 75.1812 |
| LEAP-Seq n confirming: | 6743 |
| LEAP-Seq n nonconfirming: | 2226 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACTGCTGCGATGTATTC |
| Suggested primer 2: | GGAAATCTGGATGGCTGAAA |