| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049099 |
| Chromosome: | chromosome 16 |
| Location: | 5523555 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g680500 | EFH10 | (1 of 38) IPR002048//IPR011992 - EF-hand domain // EF-hand domain pair; EF-hand Calcium binding protein, may be centrin-related | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTACTGGGTCCATATCCGCCGCCGACC |
| Internal bar code: | GGTTAGTGCGAGGTTAAAACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 98.4252 |
| LEAP-Seq n confirming: | 3375 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAGCAAGAGCGGTGTCAAT |
| Suggested primer 2: | CCAACGTTGCTGACATTCAT |