| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049113 |
| Chromosome: | chromosome 2 |
| Location: | 562232 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077550 | PHC62 | Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTAGCAGCATGATGCGCGCGCCGGCGC |
| Internal bar code: | AGCGAGGAGGGGTAACGGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 98.3128 |
| LEAP-Seq n confirming: | 3904 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAAACGATCCCCGAAGG |
| Suggested primer 2: | ACGGTCCATGATATCCCAAA |