| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.049127 |
| Chromosome: | chromosome 3 |
| Location: | 8193277 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200150 | (1 of 1) PTHR15139:SF0 - TUBULIN-SPECIFIC CHAPERONE C | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGATTGCTGACTGTTGGGGTCTGGGACAC |
| Internal bar code: | AGGGTTCTACGGGGTGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 727 |
| LEAP-Seq percent confirming: | 80.7659 |
| LEAP-Seq n confirming: | 1877 |
| LEAP-Seq n nonconfirming: | 447 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTTAAGCAATGCATGAAA |
| Suggested primer 2: | TGTCTAGTGCTGCCAAATGC |