| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049173 |
| Chromosome: | chromosome 12 |
| Location: | 5320738 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g529250 | (1 of 8) PTHR11339//PTHR11339:SF290 - EXTRACELLULAR MATRIX GLYCOPROTEIN RELATED // PROTEIN T26A8.1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGATGGAGCGAGTGAGTGAATCGGAGA |
| Internal bar code: | GGCCAGGGCGTTGCTACCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 93.663 |
| LEAP-Seq n confirming: | 1951 |
| LEAP-Seq n nonconfirming: | 132 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGCTGAAACTGCACAGGC |
| Suggested primer 2: | AGCCGCTGTAGCGGTTAGTA |