| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049180 |
| Chromosome: | chromosome 16 |
| Location: | 4465258 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g670754 | (1 of 1) PTHR10217//PTHR10217:SF512 - VOLTAGE AND LIGAND GATED POTASSIUM CHANNEL // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGAGGCTGTACGACAAGGGAGAGTACGT |
| Internal bar code: | TTCTCCCGACCGATTCACTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 208 |
| LEAP-Seq percent confirming: | 99.5204 |
| LEAP-Seq n confirming: | 415 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCTGTTGTTACCTCGG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |