| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049186 |
| Chromosome: | chromosome 7 |
| Location: | 1113808 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g320150 | ERJ1 | DnaJ-like protein, ER-targeted; (1 of 1) K09517 - DnaJ homolog subfamily B member 11 (DNAJB11) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGATAAGAGCAGGTGGGAGTGGAGCGGG |
| Internal bar code: | TGGACCTTATACCACCGCGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 529 |
| LEAP-Seq percent confirming: | 99.5108 |
| LEAP-Seq n confirming: | 1424 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGTCCCACAGAGCATT |
| Suggested primer 2: | CCCACATTCCCTCTGAACAT |