| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049193 |
| Chromosome: | chromosome 13 |
| Location: | 1239178 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g570400 | (1 of 3) 3.1.1.14 - Chlorophyllase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATGTGCGGGCTTCCGTTTCATTTCACACC |
| Internal bar code: | GGTTGGCACACGGGGTGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 76.7825 |
| LEAP-Seq n confirming: | 8486 |
| LEAP-Seq n nonconfirming: | 2566 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGAAGACGAAGAGGTGG |
| Suggested primer 2: | GGCGCCTTTTGTGTATGTTT |