| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049199 |
| Chromosome: | chromosome 10 |
| Location: | 5983203 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462600 | PUS15 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCATTGGGTGGGTGGGCGGTTACATTC |
| Internal bar code: | GTCTCACTGCTGTAGAATTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 230 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACTGCAAGGACTGGTG |
| Suggested primer 2: | CTCGAGCATAATCGGCTTTC |