| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049281 |
| Chromosome: | chromosome 9 |
| Location: | 3341303 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388504 | (1 of 16) IPR003903 - Ubiquitin interacting motif | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATAATTGCACCGTTTTGCTAGGAAGGTAT |
| Internal bar code: | AAGTTTTGAATGGCGCTTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1262 |
| LEAP-Seq percent confirming: | 99.7407 |
| LEAP-Seq n confirming: | 1923 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACAAGTACCGTGAGCGAG |
| Suggested primer 2: | CCAAGGCGTGACAAGGTAAT |