| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049344 |
| Chromosome: | chromosome 17 |
| Location: | 2286598 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713850 | SAT2,DIV184 | (1 of 1) PTHR23416:SF63 - SERINE ACETYLTRANSFERASE 2-RELATED; Serine O-acetyl transferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGAAGCCGGCGCCTGAGTATGAGATTT |
| Internal bar code: | TGACCGGGGTATGCCCCATTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 569 |
| LEAP-Seq percent confirming: | 99.3692 |
| LEAP-Seq n confirming: | 2048 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCCAAAGAAGGAGTCAGC |
| Suggested primer 2: | TGCTTTTGTGTCTACGCAGG |