| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.049352 |
| Chromosome: | chromosome 13 |
| Location: | 2903058 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583600 | DGD1 | Digalactosyldiacylglycerol synthase; (1 of 1) 2.4.1.241 - Digalactosyldiacylglycerol synthase / UDP-galactose:MGDG galactosyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAATTGAGCTAGCGCCTTAATCCATGAC |
| Internal bar code: | GGTGGCATGCGAATATTCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 620 |
| LEAP-Seq percent confirming: | 99.6493 |
| LEAP-Seq n confirming: | 13353 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTTTCGCGACCAAGTCAT |
| Suggested primer 2: | TCTCCTCAACCACCTGATCC |