| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049357 |
| Chromosome: | chromosome 9 |
| Location: | 7617276 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414850 | (1 of 2) 5.4.99.9 - UDP-galactopyranose mutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGGTCACGGTCTTGGCGTCCTTGTCA |
| Internal bar code: | GCTGGGAACCCGAGGGGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGTGGACACCATCGTCAG |
| Suggested primer 2: | ACTTCGACGAATGGATCCTG |