| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049396 |
| Chromosome: | chromosome 12 |
| Location: | 5262182 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g528450 | ASO1,LASPO1 | L-aspartate oxidase; (1 of 1) 1.4.3.16 - L-aspartate oxidase / LASPO | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTACTTTACCTGACGAGTCTGTACAGT |
| Internal bar code: | AGTCGTCCCGCGACGAGAAACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 969 |
| LEAP-Seq percent confirming: | 98.3933 |
| LEAP-Seq n confirming: | 6185 |
| LEAP-Seq n nonconfirming: | 101 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATGCTTGGACTGACCCGT |
| Suggested primer 2: | TGTCGTACCCGTGGACTGTA |