| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.049430 |
| Chromosome: | chromosome 9 |
| Location: | 1989156 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394700 | RNZ1 | (1 of 6) 3.1.26.11 - Ribonuclease Z / tRNase Z; Ribonuclease Z | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCGCAATCGAACCGCGCCAGGCGCCA |
| Internal bar code: | GTGGATTCATAGAAGAGCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTTCCAAGCGGTGTAGT |
| Suggested primer 2: | GGCAAACTGGGTTTCACACT |