| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.049448 |
| Chromosome: | chromosome 17 |
| Location: | 1699788 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708700 | HTR20,HTR27 | Histone H3; (1 of 35) K11253 - histone H3 (H3) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCTATTCCCGTGCCACGGCTTCGTGG |
| Internal bar code: | GGGCAAACTGTTATGGGTCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 632 |
| LEAP-Seq percent confirming: | 99.4002 |
| LEAP-Seq n confirming: | 1160 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCGAGAGGATTATGAACA |
| Suggested primer 2: | GTTCGAGGACACCAACCTGT |