Insertion junction: LMJ.RY0402.049448_3

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.049448
Chromosome:chromosome 17
Location:1699796
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g708700 HTR20,HTR27 Histone H3; (1 of 35) K11253 - histone H3 (H3) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGAAGACTTGATGCACCGATTCTGTCGGC
Internal bar code:AAGTATTTGTGGGACTCTCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:
LEAP-Seq percent confirming:
LEAP-Seq n confirming:0
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:0

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCGAGAGGATTATGAACA
Suggested primer 2:GTTCGAGGACACCAACCTGT